Tacoma Defiance

1. Atlanta United 2
2. Austin FC II
3. Carolina Core FC
4. Chattanooga FC
5. Chicago Fire FC II
6. Colorado Rapids 2
7. Columbus Crew 2
8. Crown Legacy FC (Charlotte FC II)
9. FC Cincinnati 2
10. Houston Dynamo 2
11. Huntsville City FC
12. Inter Miami CF II
13. Los Angeles FC 2
14. Minnesota United FC II
15. New England Revolution II
16. New York City FC II
17. New York Red Bulls II
18. North Texas SC
19. Orlando City B
20. Philadelphia Union II
21. Portland Timbers 2
22. Real Monarchs SLC
23. Sporting Kansas City II
24. St Louis CITY2
25. Tacoma Defiance
26. The Town FC
27. Toronto FC II
28. Vancouver Whitecaps FC II
29. Ventura County FC