-
Atlanta United 2
-
Austin FC II
-
Carolina Core FC
-
Chattanooga FC
-
Chicago Fire FC II
-
Colorado Rapids 2
-
Columbus Crew 2
-
Connecticut United FC
-
Crown Legacy FC (Charlotte FC II)
-
FC Cincinnati 2
-
Houston Dynamo 2
-
Huntsville City FC
-
Inter Miami CF II
-
Los Angeles FC 2
-
Minnesota United FC II
-
New England Revolution II
-
New York City FC II
-
New York Red Bulls II
-
North Texas SC
-
Orlando City B
-
Philadelphia Union II
-
Portland Timbers 2
-
Real Monarchs SLC
-
San Jose Earthquakes II
-
Sporting Kansas City II
-
St Louis CITY2
-
Tacoma Defiance
-
Toronto FC II
-
Vancouver Whitecaps FC II
-
Ventura County FC
