Atlanta United 2

1. Atlanta United 2
2. Austin FC II
3. Carolina Core FC
4. Chattanooga FC
5. Chicago Fire FC II
6. Colorado Rapids 2
7. Columbus Crew 2
8. Connecticut United FC
9. Crown Legacy FC (Charlotte FC II)
10. FC Cincinnati 2
11. Houston Dynamo 2
12. Huntsville City FC
13. Inter Miami CF II
14. Los Angeles FC 2
15. Minnesota United FC II
16. New England Revolution II
17. New York City FC II
18. New York Red Bulls II
19. North Texas SC
20. Orlando City B
21. Philadelphia Union II
22. Portland Timbers 2
23. Real Monarchs SLC
24. San Jose Earthquakes II
25. Sporting Kansas City II
26. St Louis CITY2
27. Tacoma Defiance
28. Toronto FC II
29. Vancouver Whitecaps FC II
30. Ventura County FC